The Extract of Fructus Psoraleae Promotes Viability and Cartilaginous Formation of Rat Chondrocytes In Vitro

Figure 3

The effects of P-e on cell proliferation. (a and b) EdU incorporative DNA synthesis of rat chondrocytes with P-e treatment. (a) EdU staining (red) and DAPI (blue) in chondrocytes with P-e treatment for 24 h. (b) The histogram showed the percentage of EdU⁺ nuclei. Each bar represents the mean sd (); versus DMSO group (control) was accepted as statistically significant. (c) Cell cycle by flow cytometry, indicating that 22.1%, 25.2%, and 29.6% of the cells were at the S/G2 phase for DMSO (control), 0.1 μg/mL P-e, 1 μg/mL P-e, respectively. (d) Statistical analyses for flow cytometry (); versus DMSO group (control) was accepted as statistically significant.

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