Preparative Purification of Bioactive Compounds from Flos Chrysanthemi Indici and Evaluation of Its Antiosteoporosis Effect

Figure 2

HSCCC chromatograms of ethyl acetate fraction from Flos Chrysanthemi Indici. Separation by gradient solvent system -hexane-chloroform-methanol-water (0.5 : 4 : 3 : 2, v/v/v/v) to chloroform-methanol-water (4 : 3 : 2, v/v/v) using TBE-300C with the following conditions: elution mode: head-to-tail; flow rate: 5.0 mL/min; revolution speed: 850 rpm; column temperature: 25°C; was 69.6%; stationary phase: upper phase; mobile phase: lower phase; detection: 340 nm. Peaks: acacetin (1), apigenin (2), luteolin (3), and linarin (4).