Research Article

iTRAQ-Based Proteomic Analysis of Ginsenoside F2 on Human Gastric Carcinoma Cells SGC7901

Table 2

Differentially downregulated (<0.83-fold) proteins identified by iTRAQ in F2 treated SGC7901 cells.

Rank #AccessionGene symbol (GN)Definition (description)ScoreMassCov%RationCOG function-description

Down 1tr|F5H740VDAC3 Voltage-dependent anion-selective channel protein 3 11143959841.50.81
Down 2sp|Q9H845ACAD9Acyl-CoA dehydrogenase family member 9, mitochondrial3118151221.90.69 Acyl-CoA dehydrogenases
Down 3sp|Q969S9-2GFM2Isoform 2 of ribosome-releasing factor 2, mitochondrial153940595.10.80 Translation elongation factors (GTPases)
Down 4sp|P35908KRT2Keratin, type II cytoskeletal 2 epidermal3387663018.20.67 Myosin heavy chain
Down 5tr|B7Z8A2cDNA FLJ51671, highly similar to prenylcysteine oxidase (EC 1.8.3.5)4926374023.80.83
Down 6sp|Q9Y512SAMM50Sorting and assembly machinery component 50 homolog1705933918.60.76 Outer membrane protein/protective antigen OMA87
Down 7sp|Q6ZNW5GDPGP1GDP-D-glucose phosphorylase 1 118453028.60.78
Down 8sp|P51970NDUFA8NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 722572015.10.68
Down 9tr|B4DRW0cDNA FLJ58125, highly similar to copper-transporting ATPase 1 (EC 3.6.3.4) 102618736.10.78 Cation transport ATPase
Down 10tr|Q8NBW7KDELR1ER lumen protein retaining receptor 512032712.70.73 ER lumen protein retaining receptor
Down 11tr|B2R6F5cDNA, FLJ92928, highly similar to Homo sapiens retinitis pigmentosa 2 (X-linked recessive) (RP2), mRNA 59474512.30.82
Down 12tr|Q2VIN3RBM1 (fragment) 12324575626.80.81 RNA-binding proteins (RRM domain)
Down 13sp|P14174Macrophage migration inhibitory factor 6081385617.40.71
Down 14tr|B2R6S4cDNA, FLJ93089, highly similar to Homo sapiens NCK adaptor protein 1 (NCK1), mRNA1375375518.30.83
Down 15sp|Q16822PCK2Phosphoenolpyruvate carboxykinase [GTP], mitochondrial17957878441.60.74 Phosphoenolpyruvate carboxykinase (GTP)
Down 16tr|E9PM12TCIRG1V-type proton ATPase 116 kDa subunit a isoform 3 (fragment) 632581513.30.74 Archaeal/vacuolar-type H+-ATPase subunit I
Down 17sp|Q2T9J0-2TYSND1Isoform 2 of peroxisomal leader peptide-processing protease 96436189.80.67
Down 18tr|J3KPX7PHB2Prohibitin-2 15433946651.80.82 Membrane protease subunits, stomatin/prohibitin homologs
Down 19tr|Q8NCF7cDNA FLJ90278 fis, clone NT2RP1000325, highly similar to phosphate carrier protein, mitochondrial precursor 5174857626.90.81
Down 20tr|B4E0R0cDNA FLJ54220, highly similar to Long-chain-fatty-acid-CoA ligase 1 (EC 6.2.1.3)100885606.20.74 Long-chain acyl-CoA synthetases (AMP-forming)
Down 21tr|B3KRY3cDNA FLJ35079 fis, clone PLACE6005283, highly similar to lysosome-associated membrane glycoprotein 13194885111.10.79
Down 22tr|B3KU09cDNA FLJ39034 fis, clone NT2RP7008085, highly similar to Homo sapiens ring finger protein 123 (RNF123), mRNA 1101660292.40.78
Down 23sp|Q9BVV7TIMM21 Mitochondrial import inner membrane translocase subunit Tim21 863521913.70.82
Down 24sp|Q9UMY1NOL7Nucleolar protein 71483950412.50.78
Down 25sp|Q9UNN8PROCREndothelial protein C receptor 1032790915.10.80
Down 26sp|Q86SF2GALNT7N-Acetylgalactosaminyltransferase 7 95894109.90.81
Down 27tr|I3L0U2PRSS21Testisin (fragment) 1152708314.70.82 Secreted trypsin-like serine protease
Down 28tr|B7ZLP5SAFBSAFB protein557121835130.83
Down 29tr|F2Z3N7TMEM106B Transmembrane protein 106B 1351297512.50.82
Down 30tr|B7Z361Reticulon1662783812.20.76
Down 31tr|H0Y6F2PRR5Proline-rich protein 5 (fragment) 57399292.30.78
Down 32sp|Q7Z7E8UBE2Q1Ubiquitin-conjugating enzyme E2 Q1 92547111.90.76
Down 33tr|A8K4K9cDNA FLJ76169146420078.80.83
Down 34sp|P13645KRT10Keratin, type I cytoskeletal 10 3826632121.60.55
Down 35sp|Q8N5K1CISD2CDGSH iron-sulfur domain-containing protein 2 1672036426.70.81
Down 36sp|Q8NI27THOC2THO complex subunit 2 2822417328.70.83
Down 37tr|B4DEP8cDNA FLJ56960, highly similar to Homo sapiens phosphatidylinositol 4-kinase type II (PI4KII), mRNA 127617119.80.76
Down 38sp|Q5BKZ1ZNF326DBIRD complex subunit ZNF326 145781237.90.78
Down 39tr|Q8IW24EXOC5Exocyst complex component 5 108999629.30.82
Down 40tr|B3KMG6cDNA FLJ10939 fis, clone OVARC1001065, highly similar to Homo sapiens MTERF domain containing 1 (MTERFD1), mRNA 117432259.80.76
Down 41sp|Q8NBM4-2UBAC2Isoform 2 of ubiquitin-associated domain-containing protein 2 1503730618.10.83
Down 42sp|Q8NGA1OR1M1Olfactory receptor 1M1 76395122.20.69
Down 43tr|E9PN17ATP5LATP synthase subunit g, mitochondrial 3661148963.20.82
Down 44tr|B2R686TGOLN2Trans-golgi network protein 2, isoform CRA_a 16661093130.79
Down 45tr|B4DIR5cDNA FLJ56026511437281.70.74
Down 46tr|J3KS15ICT1Peptidyl-tRNA hydrolase ICT1, mitochondrial (fragment) 16926740260.82 Protein chain release factor B
Down 47tr|F5H0F9ANAPC5Anaphase-promoting complex subunit 5 72983007.50.82
Down 48tr|C8C504HBBBeta-globin 12332005629.90.21
Down 49tr|B2R921cDNA, FLJ94171, highly similar to Homo sapiens solute carrier family 25 (mitochondrial carrier; ornithine transporter) member 15 (SLC25A15), nuclear gene encoding mitochondrial protein, mRNA533930890.77
Down 50sp|Q9Y613FHOD1FH1/FH2 domain-containing protein 1 2551416258.80.81
Down 51sp|Q92643PIGKGPI-anchor transamidase1105159210.90.77 Glycosylphosphatidylinositol transamidase (GPIT), subunit GPI8
Down 52tr|A4FTY4TXNRD2TXNRD2 protein3314167224.60.79 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes
Down 53tr|D3DP46SPCS3Signal peptidase complex subunit 3 homolog (S. cerevisiae), isoform CRA_a1472400718.90.82
Down 54sp|Q9Y5Q9GTF3C3General transcription factor 3C polypeptide 3 1541172167.80.79
Down 55sp|P60468SEC61B Protein transport protein Sec61 subunit beta 1921154637.50.72
Down 56sp|Q5RI15-2Isoform 2 of cytochrome c oxidase protein 20 homolog 10617682200.83
Down 57sp|Q9P206-2Isoform 2 of uncharacterized protein KIAA1522 1461286026.50.73
Down 58sp|Q86YN1DOLPP1Dolichyldiphosphatase 1 64289535.50.69 Membrane-associated phospholipid phosphatase
Down 59sp|O00165-2Isoform 2 of HCLS1-associated protein X-1 11134281160.81
Down 60tr|B4E303cDNA FLJ57449, highly similar to Notchless homolog 1 1275413416.50.82 FOG: WD40 repeat
Down 61sp|O00194RAB27BRas-related protein Rab-27B 562968814.20.77 GTPase SAR1 and related small G proteins
Down 62tr|B4DI41MBD1 Methyl-CpG-binding domain protein 1 72874091.80.80
Down 63tr|B0UXB6ABHD16AAbhydrolase domain-containing protein 16A 1297327510.30.83 Hydrolases of the alpha/beta superfamily
Down 64sp|Q5T8D3-2Isoform 2 of Acyl-CoA-binding domain-containing protein 5 1486435311.60.72 Acyl-CoA-binding protein
Down 65tr|B4DNZ6GTF2H3General transcription factor IIH subunit 3 48370204.50.79 RNA polymerase II transcription initiation/nucleotide excision repair factor TFIIH, subunit TFB4
Down 66sp|Q96FQ6S100A16 Protein S100-A16 3461519722.30.83
Down 67tr|B4DSE1cDNA FLJ55364, highly similar to CRSP complex subunit 6 55845243.70.73
Down 68tr|J3KNX9MYO18A Unconventional myosin-XVIIIa 1572822573.50.72 Myosin heavy chain
Down 69tr|B4DMK6cDNA FLJ60055, highly similar to Rattus norvegicus Ssu72 RNA polymerase II CTD phosphatase homolog, mRNA512374513.50.82 RNA polymerase II-interacting protein involved in transcription start site selection
Down 70tr|G3V1A0TRAPPC4HCG38438, isoform CRA_b 511483820.50.81
Down 71tr|B1AHA8HMOX1 Heme oxygenase 1 (fragment) 532552515.50.83 Heme oxygenase
Down 72sp|Q9Y3B3-2TMED7Isoform 2 of transmembrane emp24 domain-containing protein 71932490828.20.82
Down 73tr|G3V1U5GOLT1B Golgi transport 1 homolog B (S. cerevisiae), isoform CRA_c 167912120.30.77 Membrane protein involved in Golgi transport
Down 74tr|B1PBA3SKNY protein 1481094408.40.81
Down 75sp|Q15061WDR43 WD repeat-containing protein 43 138913275.60.83 FOG: WD40 repeat
Down 76tr|D3DUJ0AFG3L2AFG3 ATPase family gene 3-like 2 (yeast), isoform CRA_a (fragment)69510384221.20.83 ATP-dependent Zn proteases
Down 77tr|B2RBL9cDNA, FLJ95582, highly similar to Homo sapiens breast cancer antiestrogen resistance 1 (BCAR1), mRNA20410422360.79
Down 78sp|Q3SXM5-2Isoform 2 of inactive hydroxysteroid dehydrogenase-like protein 11703549913.50.83 Short-chain dehydrogenases of various substrate specificities
Down 79sp|O43920NDUFS5NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 1061638811.30.74
Down 80tr|H0YG20MAN1B1 Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase (fragment) 155908168.20.80
Down 81tr|Q0KKI6Immunoglobulin light chain (fragment) 66285598.20.80
Down 82sp|P62244RPS15A40S ribosomal protein S15a15211859466.20.82 Ribosomal protein S8
Down 83tr|B4DL07cDNA FLJ53353, highly similar to ATP-binding cassette subfamily D member 3 3989266916.70.81 ABC-type uncharacterized transport system, permease, and ATPase components
Down 84tr|B4DR67ALG5Dolichyl-phosphate beta-glucosyltransferase663221310.90.81 Glycosyltransferases involved in cell wall biogenesis
Down 85tr|Q9BTT5Similar to NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9 (39 kD) (fragment)18945471210.75 Predicted nucleoside-diphosphate-sugar epimerases
Down 86tr|Q5U0H8Myelin protein zero-like 1 55347254.80.74
Down 87sp|Q5SY16NOL9 Polynucleotide 5-hydroxyl-kinase NOL9 109917827.40.79 Predicted GTPase or GTP-binding protein
Down 88sp|O15173-2PGRMC2Isoform 2 of membrane-associated progesterone receptor component 2 6203016626.30.75
Down 89sp|Q5VT52-3RPRD2Isoform 3 of regulation of nuclear pre-mRNA domain-containing protein 2 2951778794.50.82
Down 90sp|Q8TC12RDH11 Retinol dehydrogenase 11 4944123814.50.76 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)
Down 91tr|B4DZ55cDNA FLJ52097, weakly similar to Homo sapiens transmembrane and tetratricopeptide repeat containing 1 (TMTC1), mRNA 16412687510.10.79 FOG: TPR repeat
Down 92tr|J3KQA9MTUS2 Microtubule-associated tumor suppressor candidate 2 1501813830.60.77
Down 93sp|Q96MG7NDNL2Melanoma-associated antigen G158416457.60.72
Down 94tr|H3BQH3KLHDC4 Kelch domain-containing protein 4 (fragment)1074735910.70.83
Down 95tr|J3KN00NDUFA13NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13 2582859923.30.81
Down 96sp|Q8NF37LPCAT1Lysophosphatidylcholine acyltransferase 1 7086734615.70.82 1-Acyl-sn-glycerol-3-phosphate acyltransferase
Down 97sp|Q9Y5P4-2COL4A3BPIsoform 2 of collagen type IV alpha-3-binding protein 82811216.70.80
Down 98tr|Q5T8U5SURF4 Surfeit 4 4182286339.80.81 Predicted membrane protein
Down 99sp|P26599-2PTBP1Isoform 2 of polypyrimidine tract-binding protein 1 5706951516.20.82
Down 100sp|Q8NC56LEMD2 LEM domain-containing protein 2 137634237.40.76
Down 101tr|Q2Q9H2G6PD Glucose-6-phosphate 1-dehydrogenase (fragment)21656431558.30.80 Glucose-6-phosphate 1-dehydrogenase
Down 102sp|P21796VDAC1Voltage-dependent anion-selective channel protein 1 23403877762.90.80
Down 103tr|J3KNH7SENP3Sentrin-specific protease 3 88739867.70.78 Protease, Ulp1 family
Down 104sp|A6NHL2-2TUBAL3Isoform 2 of tubulin alpha chain-like 3 7685128711.80.79 Tubulin
Down 105tr|B4DR71cDNA FLJ57078, highly similar to Homo sapiens opioid receptor, sigma 1 (OPRS1), transcript variant 1, mRNA 63181518.40.83
Down 106sp|Q5JRA6-2MIA3Isoform 2 of melanoma inhibitory activity protein 3 4152493697.80.80
Down 107tr|J9ZVQ3APOE Apolipoprotein E (fragment) 1713054312.20.79
Down 108tr|G5E9V5MRPS2228S ribosomal protein S22, mitochondrial2244926417.30.77
Down 109tr|B7Z7X8ATL2Atlastin-2 1127666810.80.82
Down 110sp|P54709ATP1B3 Sodium/potassium-transporting ATPase subunit beta-3 2433913517.90.83
Down 111tr|Q6IBK3SCAMP2SCAMP2 protein 258391559.70.81
Down 112tr|A4LAA3ATRXAlpha thalassemia/mental retardation syndrome X-linked1293746042.50.81 Superfamily II DNA/RNA helicases, SNF2 family
Down 113sp|Q9UK59DBR1Lariat debranching enzyme 2037218214.50.80
Down 114tr|B4DI61cDNA FLJ58182, highly similar to protein CYR61 68504146.40.70
Down 115tr|H3BNF1CLN6Ceroid-lipofuscinosis neuronal protein 6 30012918200.80
Down 116tr|E7ERK9EIF2B4Translation initiation factor eIF-2B subunit delta 170711998.80.79 Translation initiation factor 2B subunit, eIF-2B alpha/beta/delta family
Down 117tr|H0Y8C3MTCH1Mitochondrial carrier homolog 1 (fragment) 975096412.90.81
Down 118tr|B2RMV2CYTSACYTSA protein521495392.50.79 Ca2+-binding actin-bundling protein fimbrin/plastin (EF-hand superfamily)
Down 119tr|I3L1P8SLC25A11Mitochondrial 2-oxoglutarate/malate carrier protein (fragment) 4703720035.50.83
Down 120sp|Q8NBU5-2ATAD1Isoform 2 of ATPase family AAA domain-containing protein 1 1244046811.10.72 ATPases of the AAA+ class
Down 121sp|Q9Y3E7CHMP3Charged multivesicular body protein 3 1023241514.40.83 Conserved protein implicated in secretion
Down 122sp|P02763ORM1 Alpha-1-acid glycoprotein 1 2622828820.40.80
Down 123tr|Q53F51FGF intracellular binding protein isoform b variant (fragment) 16548798120.83
Down 124sp|Q3ZAQ7VMA21Vacuolar ATPase assembly integral membrane protein VMA21 2411286824.80.81
Down 125tr|B2R6X8cDNA, FLJ93169, highly similar to Homo sapiens GPAA1P anchor attachment protein 1 homolog (yeast) (GPAA1), mRNA106721517.60.80
Down 126sp|Q9P0S9TMEM14C Transmembrane protein 14C 45127748.90.70
Down 127sp|P08779KRT16Keratin, type I cytoskeletal 166305705423.90.62
Down 128sp|Q86UT6-2NLRX1Isoform 2 of NLR family member X1 751103094.10.71
Down 129tr|Q59E99Thrombospondin 1 variant (fragment) 1531557893.40.68
Down 130sp|Q8WXH0-2SYNE2Isoform 2 of nesprin-2 1499867581.10.82 Ca2+-binding actin-bundling protein fimbrin/plastin (EF-hand superfamily)
Down 131sp|P78310-2CXADRIsoform 2 of coxsackievirus and adenovirus receptor 47474913.80.74
Down 132tr|B2R995Malic enzyme 98777385.80.83 Malic enzyme
Down 133tr|Q5QP56BCL2L1Bcl-2-like protein 1 (fragment) 982181023.20.82
Down 134tr|H0YK72SEC11ASEC11-like 1 (S. cerevisiae), isoform CRA_a 2472201816.50.81 Signal peptidase I
Down 135tr|B4DDH8cDNA FLJ55184, highly similar to Homo sapiens leukocyte receptor cluster (LRC) member 4 (LENG4), mRNA 137548658.80.79 Predicted membrane protein
Down 136sp|Q9UJS0-2SLC25A13Isoform 2 of calcium-binding mitochondrial carrier protein Aralar2 7198682417.50.82
Down 137tr|A8KAK5cDNA FLJ77399, highly similar to Homo sapiens cofactor required for Sp1 transcriptional activation, subunit 2, 150 kDa (CRSP2), mRNA 851829873.40.82
Down 138tr|H0YEF3RNASEH2CRibonuclease H2 subunit C (fragment)761885625.30.77
Down 139tr|Q5QNZ2ATP5F1ATP synthase F(0) complex subunit B1, mitochondrial 4062779447.70.82
Down 140sp|Q6UW68TMEM205Transmembrane protein 205 1652329415.90.82
Down 141tr|B3KPJ4PHC2 Polyhomeotic-like protein 2 193597649.30.79
Down 142tr|H0Y4D4ACAA13-Ketoacyl-CoA thiolase, peroxisomal (fragment) 1313021812.70.78 Acetyl-CoA acetyltransferase
Down 143tr|Q4G0F4POLRMTDNA-directed RNA polymerase1671596644.60.81 Mitochondrial DNA-directed RNA polymerase
Down 144tr|Q6FGZ3EPHX1EPHX1 protein (fragment) 5196228114.90.77 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)
Down 145tr|B4DVN1cDNA FLJ52214, highly similar to DnaJ homolog subfamily B member 6 90377408.60.70 DnaJ-class molecular chaperone with C-terminal Zn finger domain
Down 146sp|Q92667-2AKAP1A-kinase anchor protein 1, mitochondrial661119404.9
Down 147sp|O00483NDUFA4 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4 1651185546.90.83
Down 148sp|Q9NTJ5SACM1LPhosphatidylinositide phosphatase SAC1 1797747618.20.83 Phosphoinositide polyphosphatase (Sac family)
Down 149tr|B3KVC5cDNA FLJ16380 fis, clone TLIVE2002882, weakly similar to imidazolonepropionase (EC 3.5.2.7) 41535823.30.83 Imidazolonepropionase and related amidohydrolases
Down 150tr|B7ZLI5FAM98CFamily with sequence similarity 98, member C 72416969.50.68
Down 151tr|B7Z6F5YIPF1Protein YIPF1 64408662.70.61
Down 152sp|Q6NVY1-2HIBCHIsoform 2 of 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial 1014654319.20.82 Enoyl-CoA hydratase/carnitine racemase
Down 153tr|U3KQJ1POLDIP2Polymerase delta-interacting protein 2 2824639526.40.76 Uncharacterized protein affecting Mg2+/Co2+ transport
Down 154tr|D6RGZ2THOC3 THO complex subunit 31721269036.20.75
Down 155tr|A0S0T0ATP6ATP synthase subunit a 128268964.40.78 F0F1-type ATP synthase, subunit a
Down 156tr|G3V2U7ACYP1Acylphosphatase 851752014.70.80 acylphosphatases
Down 157sp|Q9ULG6-2CCPG1Isoform 2 of cell cycle progression protein 1 79933134.10.81
Down 158tr|H7BXZ6RHOT1Mitochondrial Rho GTPase 142816005.90.77 GTPase SAR1 and related small G proteins
Down 159sp|Q14151SAFB2 Scaffold attachment factor B2 461129824130.83
Down 160sp|Q96LD4TRIM47Tripartite motif-containing protein 47 138758387.80.81
Down 161tr|A8K2K2cDNA FLJ76494, highly similar to Homo sapiens GTPBP2 GTP-binding like protein 2 1376476711.70.83 GTPase