Research Article
Antiobesity Effects of Unripe Rubus coreanus Miquel and Its Constituents: An In Vitro and In Vivo Characterization of the Underlying Mechanism
Table 1
Primers and expected sizes of PCR products with each primer pair.
| | Gene | Primer sequence (5′3′) | Product size (bp) | GenBank accession number |
| | PPARγ | Forward: GACCACTCGCATTCCTTT | 459 | NM_001127330.1 | | Reverse: GGCATTGTGAGACATCCC |
| | C/EBPα | Forward: CTATCAGTCTGTCCAGCCC | 302 | NM_007678.3 | | Reverse: CTATCAGTCTGTCCAGCCC |
| | SREBP-1c | Forward: AAGGGCCAGGAGTGGGTAAAC | 226 | NM_011480.3 | | Reverse: CGCGCCGCGCCCCATTAGG |
| | FAS | Forward: CAGTTCCAGCCATGAAGAGA | 192 | NM_007988.3 | | Reverse: TTTGCTGGCAAAGAGAACAC |
| | ACC | Forward: TCTATTCGGGGTGACTTTC | 350 | NM_133360.2 | | Reverse: CTATCAGTCTGTCCAGCCC |
| | GAPDH | Forward: AGATCCACAACGGATACATT | 309 | NM_008084 | | Reverse: TCCCTCAAGATTGTCAGCAA |
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PPAR: peroxisome proliferator-activated receptor γ, C/EBP: CCAAT/enhancer binding protein , SREBP-1c: sterol regulatory element binding protein 1c, FAS: fatty acid synthase, ACC: acetyl-coenzyme A, and GAPDH: glyceraldehyde-3-phosphate dehydrogenase.
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