Shengmai San Ameliorates Myocardial Dysfunction and Fibrosis in Diabetic db/db Mice

Figure 4

Effects of SMS on myocardial fibrosis and TGF-β1 expression. To detect the role of SMS in myocardial remodeling, Masson’s trichrome staining was performed to evaluate fibrosis, the myocardial cells were stained for red, and collagenous fibers were blue. The collagen deposition was quantitatively analyzed via CVF, which equals collagen area/total area 100% (a and b). To observe the underlying mechanism involved in antifibrosis of SMS, the level of transforming growth factor-β1 (TGF-β1) protein expression in heart tissue was determined using Western blotting (3 heart tissues from each group) (c and d). Data are presented as mean ± SEM. versus Control group; versus db/db group.

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