Effects of Cymbidium Root Ethanol Extract on Atopic Dermatitis

Figure 2

Effect of Cymbidium ethanol extract (CYM) on 2,4-dinitrochlorobenzene- (DNCB-) induced atopic dermatitis- (AD-) like model. Male ICR mice were divided into three groups (non-, DNCB, and DNCB and CYM treatment, ). AD was induced by shaving dorsal skin of mice and applying a 1% DNCB solution for 4 days. After sensitization, mice were treated with 0.5% DNCB solution with or without CYM (10 mg/mL) every 2 days for a total of 6 times. Subsequently, AD-like mice were euthanized. (a) Image of mouse dorsal skin after euthanasia. (b) AD-like mouse model dorsal skin lesion conditions were evaluated to determine severity of dermatitis. Five symptomatic conditions were evaluated, erythema, pruritus/dry skin, erosion, lichenification, and edema/excoriation, and scored based on severity as follows: none = 0, mild = 1, moderate = 2, and severe = 3. Sum of individual scores was defined as dermatitis score. (c) Behavior of AD-like mice was video recorded and the number of scratching behaviors was measured. Data are mean ± standard deviation (SD, ). Days on -axis are after sensitization. , , and versus DNCB treatment.

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