Protective Effect of Psoralea corylifolia L. Seed Extract against Palmitate-Induced Neuronal Apoptosis in PC12 Cells

Figure 2

Effect of PCE on PA-induced apoptosis in PC12 cells. PC12 cells were pretreated with 50 μg/ml PCE for 6 h, followed by exposure to 0.4 mM PA for 24 h. (a) The cells were costained with FITC-Annexin V/PI and analyzed by flow cytometry to determine the population of cells in early apoptosis. Graph in the right side shows quantitative data for the percentage of early apoptotic cells (the lower right quadrant) according to treatment. (b) Expression levels of apoptosis-related proteins. Western blot analyses were performed to examine the expression of PARP, caspase-3, bax, and bcl-2. Actin was used as the internal control. The bands were quantified by Image J software. The results represent the mean ± SEM from triplicate experiments. as compared with control. as compared with PA.

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