Inductive Effect of Palmatine on Apoptosis in RAW 264.7 Cells

Figure 1

TRAP-positive cell detection with the coculture method. The differentiation to osteoclast of a RAW 264.7 cell was examined using TRAP staining in culture of a RAW 264.7 cell and an MC3T3-E1 cell for 5 days. Digital images were obtained using an optical microscope. RAW 264.7 cells, which were dyed using TRAP in the cell block section, show differentiation of a RAW 264.7 cell, cytogamy image. (a)–(f) ×100 magnification, (g)–(l) ×600 magnification. (m) The number of mature osteoclasts, multinucleated RAW 264.7 cell, per a field (quadrangle of 2 mm × 2.5 mm) was counted to quantify influence of palmatine. Asterisks indicate statistically significant differences (). Error bars denote ±SD.