Inductive Effect of Palmatine on Apoptosis in RAW 264.7 Cells

Figure 2

Cell viability for palmatine with cytotoxicity CCK-8 assays. The proliferation of cells cultured with different palmatine concentrations for 24 h or 5 days was evaluated using a CCK-8 assay. Seeded cells were incubated with or without palmatine for 24 h or 5 days. The cell viability was calculated using the following formula: cell viability (%) = [ − ]/[ − ] × 100%. The absorbance was measured at 450 nm. (a) The survival rate of MC3T3-E1 cells that were incubated in palmatine component medium for 5 days. (b) The survival rate of RAW 264.7 cells that were incubated in palmatine component medium for 5 days. (c) The survival rate of RAW 264.7 cells that were incubated in palmatine component medium for 24 h. (d) The survival rate of mature RAW 264.7 cells that were incubated in palmatine component medium for 5 days. Asterisks indicate statistically significant differences ( < 0.01, n.s., no significant difference). Error bars denote ± SD.

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