Inductive Effect of Palmatine on Apoptosis in RAW 264.7 Cells

Figure 7

Bone resorption evaluation with a CaP-coated plate. Bone resorption ability of a RAW 264.7 cell examined using a bone resorption assay kit in culture of a RAW 264.7 cell and an MC3T3-E1 cell for 5 days. The bone resorption activity was evaluated by measuring the pit formation on a CaP-coated plate and the fluorescence intensity of the conditioned medium. (a)–(l) Digital images of pit formation on a CaP-coated plate were obtained using an optical microscope. The brown pit shows the locus where a mature RAW 264.7 cell (differentiation like osteoclast) dissolved a calcium plate. (a)–(f): ×40 magnification, (g)–(l): ×400 magnification. (m) The total pit area on a CaP-coated plate. (n) The fluorescence intensity of bone resorption dependence in the conditioned medium. Asterisks indicate statistically significant differences ( < 0.05, < 0.01). Error bars denote ± SD.

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