Epigenetic Profiling of H3K4Me3 Reveals Herbal Medicine Jinfukang-Induced Epigenetic Alteration Is Involved in Anti-Lung Cancer Activity

Table 1

Primer sequences for ChIP-qPCR and RT-qPCR.

Type	Gene	GenBank	Forward (5′-3′)	Reverse (5′-3′)
ChIP-qPCR	MYC	[NM_002467]	CATTCCTGCGCTATTGACAC	AAAAACCATTCCCGTTTTCC
	EGFR	[NM_005228]	AGGGAAGCTGAGGAAGGAAC	CGGCTTCAGTTTGAGACCTG
	SUSD2	[NM_019601]	AGCCTCTGTCCATCTGTCAG	TTCTTCCCATCACCCCTAGC
	PTN	[NM_002825]	GAGCCCTCCGAGAAATCGTA	CAACAAAGGCAGACTGAGCG
	GLIS2	[NM_032575]	GGTGCCAAAGGATGTTGCTT	CACAAGGGCAGGATGGTTTG
	CCND2	[NM_0201759]	CGCGTTCCCTAGTTTCTGTC	GAAACTTGAAGGGGTGAGCG
	TM4SF4	[NM_004617]	GAGGCAGTGAGGAGCTTTTG	AAAGCAAGGGGAATGAGGGT
	BCL2A1	[NM_004049]	TATCCACATCCGGGGCAATT	ACGGCATCATTAACTGGGGA
	IL31RA	[NM_139017]	GCAGAGTGTCAGCTTGTTCC	TTGCGGACATTCACAGACAC
	WISP2	[NM_003881]	CTTGGGTCAGCTCTGCAAAG	CTGCTGTAGTTGTGAAGCCC
	TNFAIP6	[NM_007115]	TGCTTCATGGACTCTGAGCT	TTTCTGTACTGCAAGCTGGC
	TMEM158	[NM_015444]	TTTGGAAAGGCTGGGAAACG	GGGAGAGGGCACGATTAGAA
RT-qPCR	SUSD2	[NM_019601]	CATGAAGCCAGCCCTCCT	GTCGGGTGGCAGGAACAT
	CCND2	[NM_0201759]	CTCCTACTTCAAGTGCGTGC	TCGCACTTCTGTTCCTCACA
	BCL2A1	[NM_004049]	AGGTGTGTGATTGTGCCATT	AATTGCCCCGGATGTGGATA
	TMEM158	[NM_015444]	GCCTAGACTTCAGCCTGGAG	ACCAGGGTCATGAAGCAGG