RETRACTED: Ex Vivo Stromal Cell-Derived Factor 1-Mediated Differentiation of Mouse Bone Marrow Mesenchymal Stem Cells into Hepatocytes Is Enhanced by Chinese Medicine Yiguanjian Drug-Containing Serum

Figure 6

Cytokeratin-18 (CK-18) expression in differentiated BM-derived MSCs (P2). BM-MSCs were cultured in the presence of 20 ng/mL HGF + 15% normal serum (a), 20 ng/mL HGF + 50 ng/mL SDF-1 + 15% normal serum (b), 20 ng/mL HGF + 50 ng/mL SDF-1 + 20% YGJ (c), or 20% YGJ (d), and CK-18 was visualized by ICC at the indicated time points (magnification ×40: (a): (b2)-(b3) and (c): (b1)-(b2); magnification ×20: (a): (b1) and (b4), (b, c): (b3)-(b4), and (d)). CK-18 staining was observed at day 14 of culture in HGF + normal serum-treated BM-MSCs, and CK-18 staining density increased until day 28 (a). CK-18 staining was observed at day 7 of culture in HGF + SDF-1 + normal serum-treated BM-MSCs, and CK-18 staining density increased until day 28 (b). CK-18 staining was observed at day 7 of culture in HGF + SDF-1 + YGJ-treated BM-MSCs, and CK-18 staining density increased until day 28 (c). Positive staining for CK-18 was observed at day 14 in cells cultured with YGJ, and staining intensity increased until day 28 (d).

(a)

(b)

(c)

(d)