Ma Huang Tang Suppresses the Production and Expression of Inflammatory Chemokines via Downregulating STAT1 Phosphorylation in HaCaT Keratinocytes

Figure 5

MHT suppresses STAT1 activation in TNF-α and IFN-γ-stimulated HaCaT cells. (a) Phosphorylation of STAT1 was measured using cells cotreated with MHT (125, 250, or 500 μg/mL) and TNF-α and IFN-γ (each 10 ng/mL, TI) for 30 min by western blotting. (b) Cellular localization of STAT1 was analyzed by immunofluorescence staining. Cells were cotreated with MHT (500 μg/mL) and TI for 30 min on glass coverslips and incubated with anti-STAT1 and FITC-conjugated secondary antibodies. The cells were fixed in 4% (v/v) methanol-free formaldehyde solution (pH 7.4), stained with anti-STAT1 (green). The stained cells were mounted in medium containing DAPI (blue) and visualized under an Olympus FLUOVIEW FV 10i confocal microscope.

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