An Extract of Chinpi, the Dried Peel of the Citrus Fruit Unshiu, Enhances Axonal Remyelination via Promoting the Proliferation of Oligodendrocyte Progenitor Cells

Figure 6

(a) Immunofluorescence staining of purified OPC cultures with antibodies against Ddx54 and BrdU. At 48 hr after addition of 20 μM BrdU, cells cultured with or without hesperidin plus narirutin (10 μg/mL each) were stained with anti-Ddx54 (top row, green fluorescence) and anti-BrdU antibodies (middle row, red fluorescence). Ddx54-positive cells (arrows in top row), BrdU-positive cells (arrows in middle row), and phase contrast images (bottom row) were shown. Arrowhead indicates BrdU-negative cells. Scale bar = 20 μm. (b) Quantitation of proliferating OPCs by double immunofluorescence staining of purified OPC cultures with antibodies for OPC markers and anti-Ddx54 antibody. Top row: percentage of BrdU⁺ cells of total cells. Bottom row: the number of the cells coexpressing BrdU and various OPC markers. Selective proliferation of Ddx54⁺ cells, but not in Olig2⁺ or NG2⁺ cells, was induced by Chinpi ingredients resulting in an increase in the total number of cultured cells. Data are represented as mean ± SEM. versus control.

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