Research Article

Identification of a New Antibacterial Sulfur Compound from Raphanus sativus Seeds

Table 2

Antibacterial activity of seed extract fractions tested against E. coli determined by the agar well diffusion method. Each value is the mean ± standard deviation of three replicates.

Fraction (10 mg/well)Inhibition zone (mm)

Ethyl acetate27 ± 2
Hexane12.5 ± 1
Water (remaining fraction)
Chloramphenicol27 ± 2

10 mg of dried extract was dissolved in 100 μL of 15% methanol and the whole volume added to the well. —: no inhibition.
Inhibitory zones in mm, including diameter of the well (6.0 mm).
15 μL of 10 mg/mL was added to each well.