Identification of a New Antibacterial Sulfur Compound from Raphanus sativus Seeds

Table 3

Antibacterial activity tested against E. coli of the eight ethyl acetate fractions separated using silica gel column chromatography and determined using the agar well diffusion method. Each value is the mean ± standard deviation from three replicates.

Fraction (2 mg/well)	Inhibition zone (mm)
Fraction 1	22 ± 1
Fraction 2	26 ± 1
Fractions 3–8	—
Chloramphenicol	27 ± 2

2 mg of dried fraction was dissolved in 100 μL of 15% methanol and the whole volume added to the well.
Inhibitory zones in mm, including diameter of the well (6.0 mm). —: no inhibition.
15 μL of 10 mg/mL was added to each well.