Vascular Protective Role of Samul-Tang in HUVECs: Involvement of Nrf2/HO-1 and NO

Figure 4

Effects of SMT on TNF-α induced intracellular ROS production in HUVECs. Cells were treated with TNF-α (50 ng/mL) for 6 hours in the absence or pretreatment of SMT (10, 30, and 50 μg/mL) for 30 min and then treated with H₂DCFDA. NAC (N-acetyl-L-cysteine) was used as ROS scavenger. Bar represents the mean ± SEM of more than 3 independent experiments. versus con group. versus TNF-α group.