Identification of Centella asiatica’s Effective Ingredients for Inducing the Neuronal Differentiation

Figure 3

75-4 is the most effective fraction for inducing neurofilament expression of cultured PC12 cells. (a) Cultured PC12 cells were treated with 50-8, 50-9, 75-1, 75-2, and 75-4 at the same dilution ratio for 72 h. The cell lysates were collected to determine the expressions of NF68. NGF (50 ng/mL) served as the positive control. GAPDH served as loading control. (b) Quantification plot is shown in histograms. Values are expressed as the percentage of increase to basal reading (untreated culture), mean ± SEM. , pooled from five independent experiments.

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