Maoto, a Traditional Japanese Herbal Medicine, Inhibits Uncoating of Influenza Virus

Figure 6

Endosomal acidification was inhibited by maoto. A549 cells were incubated with PR8 for 1 hr at 4°C (moi = 1), washed by PBS, and then cultured at 37°C with or without maoto in the presence of LysoSensor green (a) or Acridine orange (b). Acidified endosomes and lysosomes at 0, 30, and 60 min were visualized by fluorescence microscopy. The former can be visualized as green fluorescence and the latter as orange fluorescence in high concentration whereas it was green in low concentration.

(a)

(b)