An Exploration of Traditional Chinese Medicinal Plants with Anti-Inflammatory Activities

Figure 3

Inhibitory effects of plant extracts (50 μg/mL, 12.5 μg/mL) on LPS-induced mRNA expression of proinflammatory cytokines in RAW 264.7 macrophages. RAW 264.7 cells were treated with solvent vehicle (DMSO, 0.1%), positive control (parthenolide, 10 μM), or plant extract samples (50 μg/mL, 12.5 μg/mL) 1 h prior to the addition of LPS (100 ng/mL), and cells were further incubated for 4 h. The numbers were used to abbreviate plant names (see Table 1 for full names). The mRNA levels of TNF-α (a), IL-6 (b), and IL-1β (c) were determined by quantitative Real-Time PCR. Data points represent mean ± SEM; , (ANOVA) compared with negative control.

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