Long-Term Effects of TCM Yangqing Kangxian Formula on Bleomycin-Induced Pulmonary Fibrosis in Rats via Regulating Nuclear Factor-κB Signaling

Table 3

The primer sequences of mRNAs.

Gene		Oligonucleotide primers (5′-3′)	Product size (bp)	Annealing (°C)	Cycle
TNF-α	F	CGTCAGCCGATTTGCCATTT	88	60	40
	R	TCCCTCAGGGGTGTCCTTAG		60	40
IFN-γ	F	GAGGAACTGGCAAAAGGACG	132	60	40
	R	AGGTGCGATTCGATGACACT		60	40
GADPH	F	AAGGTCGGTGTGAACGGATT	70	60	40
	R	CTTTGTCACAAGAGAAGGCAGC		60	40

Note. F: forward; R: reverse.