Anticancer Effect of Nemopilema nomurai Jellyfish Venom on HepG2 Cells and a Tumor Xenograft Animal Model

Figure 3

Effects of NnV on PI3K/Akt pathway in HepG2 cells. (a) The levels of p-PI3K, p-PTEN, p-PDK1, and p-Akt were analyzed by western blotting using specific monoclonal antibodies. GAPDH was used as a loading control. (b) The levels of p-mTOR, p-p70S6K, p-S6K, p-4E-BP1, and p-eIF4E were analyzed by western blotting using specific monoclonal antibodies. (c) HepG2 cells were pretreated with LY294002 (20 μM) for 1 h and then treated with NnV.

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