DJC Suppresses Advanced Glycation End Products-Induced JAK-STAT Signaling and ROS in Mesangial Cells

Figure 1

DJC reversed the upregulated iNOS and COX2 in GMCs induced by AGEs. (a) Cells were treated with AGEs (250 g/ml) for 0, 2, 4, 6, 12, 24, 48, and 72 h. Total cell lysates from GMCs were subjected to analysis for iNOS and COX2 protein levels by western blot. (b) Laser densitometry of the gels showed in (a) and two additional experiments. (c) Total cell lysates from cells treated with AGEs or DJC in the presence of AGEs for 48 h were subjected to analysis for iNOS and COX2 protein levels. (d) Laser densitometry of the gels showed in (c) and two additional experiments. β-Actin expression acted as an internal reference. The columns and error bars represent the mean and SD ( per group). P < 0.05, P < 0.01, and P < 0.001 versus Ctrl; P < 0.05, P < 0.01, and P < 0.001 versus AGEs. Similar results were obtained in at least three independent experiments.