DJC Suppresses Advanced Glycation End Products-Induced JAK-STAT Signaling and ROS in Mesangial Cells

Figure 2

DJC suppressed STAT-responsive inflammatory gene expression. Cells were treated with DJC (0.125, 0.5, and 2 mg/ml) or AG490 (20 μM) in the presence of AGEs (250 μg/ml) for 48 h. The contents of TNF-α, IL-6, and MCP-1 in cell culture medium were determined using double-antibody sandwich ELISA. Data are presented as mean ± SD. The columns and error bars represent the mean and SD ( per group). P < 0.05, P < 0.01, and P < 0.001 versus Ctrl; P < 0.05 and P < 0.01 versus AGEs. Similar results were obtained in at least three independent experiments.