Compound Wumei Powder Inhibits the Invasion and Metastasis of Gastric Cancer via Cox-2/PGE2-PI3K/AKT/GSK3β/β-Catenin Signaling Pathway

Figure 6

Cox-2/PGE2 promotes the invasion and migration and regulates PI3K/AKT/GSK3β/β-catenin signaling pathway. (a) Observation of cell invasion after the SGC-7901 cells were treated with PGE2 and Celecoxib. (b) The histogram shows the number of SGC-7901 cells passing through the 8 μm pore size into the lower chamber. (c) Observation of cell migration after the cells were treated with PGE2 and Celecoxib. (d) The histogram shows the percentage of wound healing in SGC-7901 cells after administration. (e) Extracts from SGC-7901 cells treated with PGE2 and Celecoxib were analyzed for the expression of p-AKT, AKT, p-GSK3β, GSK3β, β-catenin, and nuclear β-catenin. (f) The histogram shows the relative expression of each protein in (e). (g) The histogram shows the relative ratios of p-AKT/AKT and p-GSK3β/GSK3β in each group. Data are expressed as the mean ± SD of three experiments ( < 0.05 compared with control group; < 0.01 compared with control group).

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