A New Technique Using Low Volumes: A New Technique to Assess the Molluscicidal Activity Using Low Volumes

Figure 1

Molluscicidal evaluation models with niclosamide on Biomphalaria glabrata under laboratory conditions. (a) Becker of 1000 mL, containing the control groups, water, and 1% aqueous dimethylsulfoxide, 500 mL of aqueous solutions of niclosamide (1 mg/L, 2 mg/L, 3 mg/L, 4 mg/L, and 5 mg/L), and 10 Biomphalaria glabrata, with a size between 10 and 12 mm in diameter by Becker, created and kept in the laboratory. (b) Methodology with 24-well plates, containing the control groups and the doses of niclosamide and 1 snail per well with 2 mL of solution. Exposure of 10 animals per dose as described in (a). (c) Methodology with 24-well plates, containing the control groups and the doses of niclosamide and 1 mollusk per well with 2 mL of solution as described above. Exposure of 3 animals per dose.

(a)

(b)

(c)