Naoxintong/PPAR Signaling Inhibits Cardiac Hypertrophy via Activation of Autophagy

Figure 4

NXT/PPARγ signaling mediates autophagy. (a) H9c2 cells were transfected with control shRNA or PPARγ shRNA plasmids. After 24 h, cells were treated with or without 0.5 μg/mL NXT for 3 h. Cell lysates were subjected to Western blot. Quantitation of LC3-II/LC3-I level was shown. Results are expressed as means ± SEM (). (b) H9c2 cells were transfected with control shRNA or PPARγ shRNA plasmids. After 24 h, cells were treated with or without 0.5 μg/mL NXT for 3 h. Cell lysates were subjected to Western blot. Quantitation of p62/SQATM1 level was shown. Results are expressed as means ± SEM (). (c) H9c2 cells were transfected with control shRNA or PPARγ shRNA plasmids. After 24 h, cells were treated with or without 0.5 μg/mL NXT for 6 h. Autophagy-associated gene expression was assayed by qPCR. Results are expressed as means ± SEM ().

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