Curcumin Induces p53-Null Hepatoma Cell Line Hep3B Apoptosis through the AKT-PTEN-FOXO4 Pathway

Figure 7

Effects of FOXO4 overexpression on curcumin induced Hep3B cell apoptosis. (a) Western blot analysis of overexpressed and endogenous FOXO4 proteins in Hep3B cells. (b) Effects of FOXO4 overexpression on Hep3B cell apoptosis with and without curcumin treatments. (c) Caspase-3 activities in FOXO4 overexpression experiments. -axis indicates propidium iodide (PI) intensities; -axis indicates caspase activities. pcDNA 3.1 is a mammalian expression vector with the CMV promoter (Invitrogen). The concentration of curcumin used in the experiments was 50 μM. The data are representative of triplicate experiments. CUR: curcumin. DMSO: dimethyl sulfoxide (solvent of curcumin).

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