trans-Cinnamaldehyde Inhibits Microglial Activation and Improves Neuronal Survival against Neuroinflammation in BV2 Microglial Cells with Lipopolysaccharide Stimulation

Figure 2

Effects of TCA on the protein levels of iNOS, COX-2, IL-1β, and TNF-α in LPS-stimulated BV2 cells. BV2 cells were pretreated with 2.5–10 μM TCA for 2 h prior to 0.1 μg/ml LPS stimulation. After 24 h, cells were lysed and cell lysates were subjected to Western blot to detect iNOS (a) and COX-2 (b) expression. The protein levels of iNOS and COX-2 were standardized based on the respective level of β-actin protein. Value was expressed as relative changes in comparison to Control, which was set to 1. Supernatants were collected at 6 h after LPS stimulation and subjected to ELISA to measure the amount of IL-1β (c) and TNF-α (d) secreted by BV2 cells with TCA 10 μM pretreatment. Each value indicates the mean ± SEM from three independent experiments. , , and versus untreated Controls; , , and versus LPS alone.

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