trans-Cinnamaldehyde Inhibits Microglial Activation and Improves Neuronal Survival against Neuroinflammation in BV2 Microglial Cells with Lipopolysaccharide Stimulation

Figure 6

TCA attenuated microglial neurotoxicity in the microglia/neuron coculture. BV2 cells were cocultured with PC12 cell using transwell system. (a) The experimental timeline for microglia/neuron coculture. BV2 cells were pretreated with or without TCA (10 μM) for 2 h and then stimulated with LPS (0.1 μg/ml) prior to coculture. (b) After 24 h of coculture period, transwell inserts were removed and MTT assay was performed to determine the viability of PC12 cells in the underwells. Data are the mean ± SEM from three independent experiments. , , and versus untreated Controls; , , and versus LPS alone.

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