Zanthoxylum heitzii Modulates Ferric Nitrilotriacetate-Dependent Oxidative Alterations in Four Vital Organs: An In Vitro Organoprotective Model

Table 1

Treatment protocol.

Reagents	Blank	Negative control	Positive control	Vitamin C	Samples
Phosphate buffer (0,1 m; pH 7,4) (µl)	680	700	680	580	580
Plant extracts [100 μg/ml] (μl)	0	0	0	0	100
Vitamin C [1 mg/ml] (µl)	0	0	0	100	0
Organ homogenates (µl)	300	300	300	300	300
Homogenisation and incubation for 1 h at 37°C
Prooxidant solution (µl)	20	0	20	20	20
Homogenisation and incubation for 1 h at 37°C

The samples were incubated in a hot water bath (37°C) and each of the columns represents a test group.