Dahuang Zhechong Pill Combined with Doxorubicin Induces Cell Death through Regulating Energy Metabolism in Human Hepatocellular Carcinoma Cells

Figure 4

Effects of DHZCP-medicated serum with or without DOX on oxidative phosphorylation enzymes involved in oxidative phosphorylation and glycolysis in SMMC-7721. (a) Western blot analyses of expression of CI, CII, CIII, CIV, CV, HK2, PFKM, and PKM2. (b) and (c) Relative expression normalized to β-actin. Groups: (1) control; (2) DHZCP-medicated serum; (3)–(5) DOX (0.5, 2, and 8 μM); (6)–(8) combination of DHZCP-medicated serum with DOX (0.5, 2, and 8 μM). Significance: , in comparison with control; , compared with DOX group.

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