Potential Protective Effects of Bioactive Constituents from Chinese Propolis against Acute Oxidative Stress Induced by Hydrogen Peroxide in Cardiac H9c2 Cells

Figure 5

Caffeate derivatives in CP decreased the H₂O₂ induced elevation of and inhibited cell apoptosis in H9c2 cardiomyocytes. (a) Effects of CP bioactive compounds (BZC, CAPE, and CNC) on intracellular induced by H₂O₂. H9c2 cells were pretreated with BZC, CAPE, and CNC for 12 h and then stimulated with 6 h H₂O₂ (700 μM). was measured as described in materials and methods. (b–d) Effects of CP bioactive compounds on cell apoptosis induced by H₂O₂. Various concentrations (1, 5, and 10 μM) of BZC (b), CAPE (c), and CNC (d) were added into cell medium for 12 h and then stimulated with 6 h H₂O₂ (700 μM). Cell apoptotis were analysed using FCM as described in Materials and Methods. versus oxidative injury group and versus control group.

(a)

(b)

(c)

(d)