5,7-Dihydroxyflavone Analogues May Regulate Lipopolysaccharide-Induced Inflammatory Responses by Suppressing IκBα-Linked Akt and ERK5 Phosphorylation in RAW 264.7 Macrophages

Figure 6

Effects of 1 or 9 with or without Akt or ERK5 inhibitor on inflammatory rerated proteins in RAW 264.7 cells. RAW 264.7 cells were seeded into a 6-well plate (3 10⁵ cells/well) and incubated for 2 hours. Then they were treated with an inhibitor of appropriate concentration for one hour followed by stimulation with LPS of 100 ng/well with or without various concentration of a dimethyl sulfoxide (DMSO) solution of a 5,7-dihydroxyflavone analogue for 15 min (IκBα, Akt, and Erk5) or 16 hours (iNOS and COX2). Labels present the compound names and concentrations (μM). Data are expressed as mean ± SD from 3 independent experiments. Control group were presented by the open circles. : and : .