Effects of the Fruit Extract of Tribulus terrestris on Skin Inflammation in Mice with Oxazolone-Induced Atopic Dermatitis through Regulation of Calcium Channels, Orai-1 and TRPV3, and Mast Cell Activation

Figure 2

Effects of TF extract on symptoms and transepidermal water loss in oxazolone-induced AD mice. TF extract and HC dissolved in propylene glycol were applied to the dorsal skin of oxazolone-induced AD mice once a day from days 18 to 24. AD-like symptoms were examined with the naked eye and representative features were recorded (a). Symptom scores were measured in all mice as follows: 0, no symptoms; 1, mild (dryness, scaling); 2, moderate (dryness, scaling, and erosion); 3, middle (dryness, scaling, erosion, and excoriation); and 4, severe (dryness, scaling, erosion, excoriation, and hemorrhage) (b). TEWL was measured in three different parts of the dorsal skin with a Vapometer for 10 seconds (c). The results are expressed as the mean ± SD ( per a group). and versus normal (A) or AD control (B) mice. Nor, normal group; AD, oxazolone-induced AD group; 1% TF, 1% TF-applied group in AD control; 1% TF + 0.1% HC, 1% TF, and 0.1% HC-applied group in AD control; and 1% HC, 1% HC-applied group in AD control ( per a group).

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(b)

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