Extract from Periostracum cicadae Inhibits Oxidative Stress and Inflammation Induced by Ultraviolet B Irradiation on HaCaT Keratinocytes

Figure 4

P. cicadae inhibits UVB-induced MMPs and IL-6 via suppression of JNK MAPK in HaCaT cells. (a) The JNK and p38 levels in cell nucleus were determined by Western blotting and β-actin was used as loading controls. The relative expression levels of JNK and p38 were compared to control cells and presented as the mean ± SD for three independent experiments performed in triplicate. (b) The expression of MMPS in the presence of SP600125 and SB203580, respectively. MMPs levels in cell were determined by Western blotting and β-actin was used as a loading control. The relative expression levels of MMP-9 and MMP-2 were compared to control cells and presented as the mean ± SD for three independent experiments performed in triplicate. (c) Effects of P. cicadae on the IL-6 in the presence of SP600125 and SB203580, respectively. The UVB-incubated HaCaT cells pretreated with signal inhibitors were incubated with P. cicadae for 24 hours. The production of IL-6 was analyzed by ELISA. The data represent at least three independent experiments. ,  , and   were considered as a significant difference compared to control group ( versus control).

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