Research Article

Antineuroinflammatory Effects of Modified Wu-Zi-Yan-Zong Prescription in β-Amyloid-Stimulated BV2 Microglia via the NF-κB and ERK/p38 MAPK Signaling Pathways

Table 3

MWP inhibits the mRNA expression of inflammatory mediators, as well as the protein expression of iNOS in Aβ-activated BV2 microglia. The PCR primer of mRNA is shown in the table.

TNF-α5′-3′ (forward) AGTGACAAGCCTGTAGCCCACGT
5′-3′ (reverse) CCATCGGCTGGCACCACTAGTT
MCP-15′-3′ (forward) CTTCTGGGCCTGCTGTTCACAGTT
5′-3′ (reverse) TTCTTGGGGTCAGCACAGACCTCT
IL-1β5′-3′ (forward) TGGAGAAGCTGTGGCAGCTACCT
5′-3′ (reverse) GAACGTCACACACCAGCAGGTT
IL-65′-3′ (forward) ACAAAGCCAGAGTCCTTCAGAGAGA
5′-3′ (reverse) TGGTCTTGGTCCTTAGCCACTCCT
GAPDH5′-3′ (forward) GGTGAAGGTCGGTGTGAACG
5′-3′ (reverse) CTCGCTCCTGGAAGATGGTG