Colquhounia Root Tablet Protects Rat Pulmonary Microvascular Endothelial Cells against TNF--Induced Injury by Upregulating the Expression of Tight Junction Proteins Claudin-5 and ZO-1

Figure 5

CRT upregulates the expression of tight junction proteins Claudin-5 and ZO-1. Rat PMEC monolayers were divided into 6 groups: normal control, TNF-α, Dex, CRT high, CRT medium, and CRT low groups. After 48 h incubation, the expression of Claudin-5 and ZO-1 mRNA (a) and protein (b) was detected by qRT-PCR and Western blot, respectively. , P<0.05, , P<0.01, TNF-α group vs. normal control group; #, P<0.05, ##, P<0.01, treatment groups vs. TNF-a group; △, P<0.05, △△, P<0.01, CRT groups vs. Dex group.

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