Colquhounia Root Tablet Protects Rat Pulmonary Microvascular Endothelial Cells against TNF--Induced Injury by Upregulating the Expression of Tight Junction Proteins Claudin-5 and ZO-1

Figure 6

Therapeutic effects of CRT were greatly reduced by siRNAs. Rat PMECs at 70% confluence were transfected with siRNAs or siNC. Cells were incubated in medium containing 500 ng/mL CRT and 10 ng/mL TNF-α. After 48 h, cells were collected and the expression of Claudin-5 and ZO-1 was determined by Western blot (a). Cell proliferation (b) and apoptosis (c) were also measured. , p < 0.05, TNF-α+CRT+si-ZO-1 or si-Claudin-5 vs. TNF-α+CRT+si-NC.