Research Article
Preclinical Evaluation of In Vitro and In Vivo Antiviral Activities of KCT-01, a New Herbal Formula against Hepatitis B Virus
Table 1
Primers used to analyze mice liver tissue mRNAs.
| | | | Forward | Reverse | Remarks |
| | 1 | mTNF- | AGGGTCTGGGCC
ATAGAACT | CCACCACGCTCTT
CTGTCTAC | |
| | 2 | mIL-6 | GACAACTTTGGC
ATTGTGG | ATGCAGGGATGA
TGTTCTG | |
| | 3 | mMCP | ATTGGGATCATCT
TGCTGGT | CCTGCTGTTCACA
GTTGCC | |
| | 4 | mIFN- | AGCTCCAAGAAA
GGACGAAC | GCCCTGTAGGTG
AGGTTGAT | |
| | 5 | 3.5 kb/pg RNA | GGTCCCCTAGAA
GAAGAACTCCCT | CATTGAGATTCCC
GAGATTGAGAT | |
| | 6 | cccDNA level | CCGTCTGTGCCTT
CTCAT | CACAGCTTGGAG
GCTTGAAC | PROBE:
CGTGTGCACTTCG
CTTCACCTCTGC |
| | 7 | small S | TTGACAAGAATC
CTCACAATACC | GGAGGTTGGGGA
CTGCGAAT | Real-SF (positions 218-240)
Real-SR (positions 309-328) |
| | 8 | HBV virion particle | TTAACAAGAATC
CTCACAATA | GGAGGTTGGGGA
CTGCGAAT | |
| | 9 | 18S rRNA | AGTCCCTGCCCTT
TGTACACA | CGATCCGAGGGC
CTCACTA | |
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