GZFLW Induces Apoptosis of Ectopic Endometrial Stromal Cells via Promoting VPS53 Protein Stability

Figure 6

Using shRNA to konck down VPS53 and validated by RT-PCR and western blot. (a) Control virus vector, shRNA-1 #, 2 #, and 3 # lentiviruses are transiently infected in Y14 cells and total RNA is collected 72 hours later for qPCR detection. (b) Screening of stable cell lines using the third shRNA (3 #), and the mRNA expression of VPS53 was detected by qPCR. .

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