Research Article

GZFLW Induces Apoptosis of Ectopic Endometrial Stromal Cells via Promoting VPS53 Protein Stability

Figure 7

GZFLW activates Caspase-3 and Caspase-9 via VPS53. (a and b) Western blot detection of activated Caspase-9 and Caspase-3 expression in shControl stable Y14 cell lines and shVPS53 stable cell lines with or without GZFLW treatment, Actin as an internal control. (c) CCK-8 assay detected the Y14 cells proliferation of three groups at 24, 48, and 72 hours, respectively. (d) Cells in three groups were stained with Annexin V-FITC and prodiduim iodine (PI) and detected by FACS. Values are means±SEM. p-values were determined by Student’s t-test, and .
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