Antiobesity Effect of Astilbe chinensis Franch. et Savet. Extract through Regulation of Adipogenesis and AMP-Activated Protein Kinase Pathways in 3T3-L1 Adipocyte and High-Fat Diet-Induced C57BL/6N Obese Mice

Figure 2

Effects of the Astilbe chinensis Franch. et Savat. (AC) extract on inhibiting 3T3-L1 adipocyte differentiation. (a) The 3T3-L1 cell proliferation was measured by MTS assay after 24 h, 48 h, 72 h, and 96 h treatment of AC extract at various concentrations. (b) The 3T3-L1 cells differentiated with differentiation medium in the absence or presence of AC extract for 8 days. Intracellular lipids were stained with Oil Red O, and triglyceride content was quantified by spectrometry at 540 nm. (c) mRNA levels of PPAR-γ, C/EBPα, SREBP1, FAS, and SCD-1, as determined by reverse transcription PCR (upper panels) and real-time PCR (lower panels), and (d) the protein expression levels of PPAR-γ, C/EBPα, SREBP1, FAS, and SCD-1 were examined by western blot analyses. Data are presented as the mean ± SEM of three independent experiments, each performed in triplicate. < 0.001 vs untreated cells; < 0.05, < 0.01 and < 0.001 vs differentiated cells (DM).

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