Antiobesity Effect of Astilbe chinensis Franch. et Savet. Extract through Regulation of Adipogenesis and AMP-Activated Protein Kinase Pathways in 3T3-L1 Adipocyte and High-Fat Diet-Induced C57BL/6N Obese Mice

Figure 4

Effects of Astilbe chinensis Franch. et Savat. (AC) extract on epididymal fat size in HFD induced obese mice. (a) The H & E stained epididymal fat tissue from HFD induced obese mice treated with RD or AC (100 and 200 mg/kg), and (b) quantification of epididymal fat tissues size were calculated. The (c) mRNA levels of PPAR-γ, C/EBPα, SREBP1, FAS, and SCD-1, as determined by reverse transcription PCR (upper panels) and real-time PCR (lower panels), and (d) the protein levels of PPAR-γ, C/EBPα, SREBP1, FAS, and SCD-1 were examined by western blot analyses, respectively. < 0.001 vs RD mice; < 0.05, < 0.01, and < 0.001 compared with HFD mice.

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