Antiobesity Effect of Astilbe chinensis Franch. et Savet. Extract through Regulation of Adipogenesis and AMP-Activated Protein Kinase Pathways in 3T3-L1 Adipocyte and High-Fat Diet-Induced C57BL/6N Obese Mice

Table 2

Primer sequence.

Gene	Primer Sequence ( → )		Annealing
	Forward primer	Reverse primer	temp (°C)
PPAR-γ	TTTTCAAGGGTGCCAGTTTC	AATCCTTGGCCCTCTGAGAT	58
C/EBPα	AGACATCAGCGCCTACATCG	TGTAGGTGCATGGTGGTCTG	58
SREBP1	GCGCTACCGGTCTTCTATCA	TGCTGCCAAAAGACAAGGG	58
FAS	GATCCTGGAACGAGAACAC	AGACTGTGGAACACGGTGGT	50
SCD-1	CGAGGGTTGGTTGTTGATCTGT	ATAGCACTGTTGGCCCTGGA	56
PGC-1α	CACCAAACCCACAGAAAACAG	GGGTCAGAGGAAGAGATAAAGTTG	60
PPARα	ATGAAGAGGGCTGAGCGTAGGTAA	TGCCGTTGTCTGTCACTGTCTGAA	57
ATGL	ACCAACACCAGCATCCAGTT	TTTGCACATCTCTCGGAGGA	56
HSL	TTCGAGGGTGATGAAGGACT	ACTCTGGGTCTATGGCGAAT	58
Actin	GTCGTACCACTGGCATTGTG	GCCATCTCCTGCTCAAAGTC	60