Quercetin Potentiates the NGF-Induced Effects in Cultured PC 12 Cells: Identification by HerboChips Showing a Binding with NGF

Figure 1

Extract of Ginkgo Folium was fractionated by HPLC and then dotted and fixed on a chip. The chip was incubated with biotinylated protein target (NGF) and then probed with streptavidin-Cy5 before fluorescence detection. (a) Adopted from Huang et al., 2015, showing the design of HerboChip, the quantifier and qualifier controls were included. (b) Scanning result of HerboChip using Ginkgo Folium extract and probed with biotinylated NGF (left) and probed with incubation buffer only (right). Representative scanned images were shown, . (c) Superimposition of the read-out of HerboChip screening and HPLC chromatogram. HPLC chromatograms (solid line) and quantified HerboChip screening read-out (dotted line with shaded area) of the extract of Ginkgo Folium were superimposed. A will resolved peak at retention time ~67 min was labelled as D1 and submitted for further analysis.

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