Toll-Like Receptor 4–Myeloid Differentiation Primary Response Gene 88 Pathway Is Involved in the Shikonin Treatment of CIA by Regulating Treg/Th17 Expression

Figure 2

(a) Expression of cytokine and transcription factor in the arthritic joint. The expression of IL-17A, ROR-γt, IL-10, TGF-β, and Fox-P3 was determined by using RT-PCR analysis. β-actin was used for loading control. Note: S-h: high dose of shikonin, S-m: medium dose of shikonin, S-l: low dose of shikonin. (b) The density histogram data of cytokine and transcription factor in the arthritic joint. The expression of IL-17A, ROR-γt, IL-10, TGF-β, and Fox-P3 was determined by using quantitative RT-PCR analysis. β-actin was used for loading control. The density histogram data were from three-separated quantitative RT-PCR analysis (mean±SE), which represents the relative expression of IL-17A, ROR-γt, IL-10, TGF-β, and Fox-P3, respectively. (c) The protein expression in the spleen. The expression of IL-17A, ROR-γt, IL-10, TGF-β, and Fox-P3 was analyzed by Western blot as described in Materials and Methods. β-actin was used for loading control. (d) The density histogram data from three-separated Western blot analysis (mean±SE), which represents the relative expression of IL-17A, ROR-γt, IL-10, TGF-β, and Fox-P3. indicates P<0.05 or indicates P<0.01 between treated and control groups.

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