Establishment and Validation of an In Vitro Screening Method for Traditional Chinese Medicine-Induced Nephrotoxicity

Figure 9

Representative micrographs of renal glomerular histology. Control group: normal glomerular morphology (a). Experimental groups: aristolochic acid (AA) (b), cisplatin (CDDP) (c, h), diosbulbin B (DB) (d), cantharidin (CA) (e), triptolide (TR) (f, i), and sophocarpine (SC) (g). Normal glomerular morphology from control animals (a) and altered glomerular morphology in drug-treated rats (b–i). Enlarged glomeruli, increased cell number, and lobulated glomeruli (b–h). Mesangial cell proliferation, increased intercellular matrix, and dilatation of Bowman’s space (thick arrows in (b)–(h)). The capillary loops are open or collapsed closed (short arrows in (b)-(g)). Red dye foam sample exudate can be seen in the renal capsule. Epithelial hyperplasia and glomerular adhesions with the renal capsule (asterisks in (b)–(g) and (i)). H&E stain. Magnification: ×10 ((h) and (i)) and ×40 (a–g).

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