Nutmeg Extract Increases Skeletal Muscle Mass in Aging Rats Partly via IGF1-AKT-mTOR Pathway and Inhibition of Autophagy

Figure 4

Nutmeg stimulates IGF1 signalling pathway and induced AKT and mTOR signalling. This induction stimulated inhibition of autophagy activity in soleus muscle. Representative immunoblot was shown (a) and densitometric quantification was shown in the graph normalized by GAPDH. Data represent ratio in mean ± SEM of experiment. p <0.05 compared with control group. Increasing level of AKT-mTOR supports that nutmeg stimulates muscle protein synthesis most probably through activation of IGF1, AKT, and mTOR pathway. The protein level of p62 and LC3BII shows that activation of IGF1, AKT, and mTOR pathway leads to inhibition of autophagy process.

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