The Role of Berberine in the Prevention of HIF-1α Activation to Alleviate Adipose Tissue Fibrosis in High-Fat-Diet-Induced Obese Mice

Figure 4

Berberine suppressed HIF-1 and adipocyte apoptosis in eWAT of HFD-induced obese mice. Western blot analysis for HIF-1α expression (n=3) (a, b); RT-qPCR analysis for relative mRNA expression of LOX (n=6) (c). Immunohistochemistry and quantitative analysis for cleaved caspase-3 (n=3) (d, e). Western blot analysis for cleaved caspase-3 expression (n=3) (f, g). Data are shown as mean ± SEM. P < 0.05 versus NC group; P < 0.01 versus NC group; P < 0.05 versus HFD group; P < 0.01 versus HFD group.

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